PRUNE1 and type 2 diabetes mellitus: Using an FDR threshold of ≤20% and log2 fold change of 1 across 80 years of life, 7 of the genes reported to be downregulated in T2D decreased with age in our study; CTNNA2, ENPP2, ARG2, O3FAR1, WNK1, IAPP and UNC5D, while two of the genes increased with age; GPM6A and PRUNE. 5 of the genes reported to be upregulated in T2D increased with age, SERPINF1, FGF7, CLDN1, ZFP36L1 and SOX9 (Fig 5B and S4 Table).