Using mRNA microarray analysis, we identified 10 genes (NEDD9, TM4SF1, IL6, TIPARP, JMJD1C, SFRS3, IER3, ZNF57, ZNF627, and DUSP10) commonly upregulated in all 3 ESCC SP cells, when compared to in their respective non-SP cells. This evidence concerns the gene SRSF3 and esophageal squamous cell carcinoma.