To explore the biological function of L1CAM in esophageal carcinoma, we used qRT-PCR to assess the L1CAM expression in 4 ESCC cell lines (EC1, KYSE70, TE1, and KYSE450) and an immortalized esophageal epithelial cell line, Het-1α. This evidence concerns the gene L1CAM and esophageal squamous cell carcinoma.