A xenograft murine model of pancreatic cancer wasdeveloped forthe in vivo analysis of the optimized AMF conditions.CALR expression, a marker of the immune response, was significantlyenhanced in those cells containing MNPs after the MH treatment, openingthe way to further studies on immune stimulation after MH using animalmodels with a complete immune system. This evidence concerns the gene CALR and pancreatic neoplasm.