CALR and neoplasm: Once the optimalAMF conditions were selected, in vivo experimentswere carried out using similar frequency and field amplitude parameters.A marker of the immune response activation, calreticulin (CALR), wasevaluated in cells from a xenograft tumor model after the MH treatment.Moreover, the distribution of nanoparticles within the tumor tissuewas assessed by histological analysis of tumor sections, observingthat the exposure to the alternating magnetic field resulted in themigration of particles toward the inner parts of the tumor.