As a negative control, no immunoreactivity of FMRpolyG was observed in a case of spinocerebellar ataxia 3 (SCA3) (Fig. 4I–L), confirming the specificity of our antibodies for the FMR1-premutation-translated product, FMRpolyG. This evidence concerns the gene FMR1 and Spinocerebellar ataxia type 3.