Among them, ANLN, BIRC5, BUB1B, CDC20, CDCA5, CDK1, NCAPG, NEK2, and TOP2A were most negatively related to overall survival (Figure 6) and disease-free survival (Figure 7) in the Kaplan–Meier survival analysis of HCC patients. This evidence concerns the gene NEK2 and hepatocellular carcinoma.