Furthermore, we found that AS-IV could downregulate the expressions of TLR4 mRNA, MyD 88 mRNA, NF-κB p65 mRNA, and their proteins in the liver tissue of HFD-induced NAFLD rats, and, meanwhile, reduce serum TNF-α, IL-6, and IL-8 levels. This evidence concerns the gene TLR4 and metabolic dysfunction-associated steatotic liver disease.