We investigated the actions of FPS and CTR on the expressions of critical signaling molecules in FGF23-Klotho signaling axis including Klotho, FGF23 and FGFR1 in renal tubulointerstitium and the kidneys of the CKD–MBD model rats by IHC staining and WB analysis. The gene discussed is FGF23; the disease is Marchiafava-Bignami disease.