As shown in Figures 3, 4, after induction of renal injury, in line with renal dysfunction, increased levels of urine Ca2+, serum P4+ and FGF23, decreased levels of urine P4+, serum Ca2+ and VD3, as well as reduced value of BMD in the femur bone and lumbar vertebrae of the CKD–MBD model rats were revealed, respectively, compared to those of the Sham group rats. This evidence concerns the gene FGF23 and Marchiafava-Bignami disease.