EWSR1 and amyotrophic lateral sclerosis: To prioritize interacting partners to ALS risk genes found in SC.M4, we leveraged a direct protein–protein interaction (PPI) network using the top 300 hub genes of SC.M4, finding a significant network that includes ALS risk genes such as TARDBP and EWSR1 (Fig. 3E, “Methods”, p = 0.001).