As noted above, we observed an unbalanced copy number gain affecting the region of chromosome 10 (nucleotides 42,356,527–135,534,747) that includes PTEN in both 2XSB cells and the parent tumor (Supplementary Table 1); this LOH was accompanied by the presence of a pathogenic missense mutation in PTEN, p.P38S (Fig. 7a). This evidence concerns the gene PTEN and neoplasm.