Of 212 patients, 145 were not subject to further multiplex RT-PCR or next-generation sequencing assays as they carried known ALL subtype genetic alterations, including hyperdiploidy, hypodiploidy, intrachromosomal amplification of chromosome 21, KMT2A rearrangement, ETV6-RUNX1, BCR-ABL1, TCF3-PBX1, TCF3-HLF and P2RY8-CRLF2. This evidence concerns the gene PBX1 and acute lymphoblastic leukemia.