Immunoblotting and RT/qPCR data demonstrated that TNFAIP8 expression was higher in SK-MEL-2 (melanoma cells), A431 (SCC cells), and HaCaT (normal skin cells) which express B-RAF wild type protein, and lower expression of TNFAIP8 was observed in A375 and A2058 melanoma cells that expressed B-RAFV600E mutant protein (Fig. 1E,F). This evidence concerns the gene BRAF and melanoma.