Interestingly, B-RAF mutant A375 and A2058 melanoma cell lines which expressed lower levels of TNFAIP8, upon a dose-dependent treatment with TNFα (10–50 ng/ml) induced TNFAIP8 protein expression (Fig. 2C) suggesting that TNFα induced TNFAIP8 expression in normal and skin cancer cell lines irrespective B-RAF background. This evidence concerns the gene TNF and melanoma.