Interestingly, in orthotopic tumor models of CRC with spontaneous metastasis by trans-anal injection (TRAI) of murine colon cancer cells submucosally into the distal and posterior rectum of mice, we found that there were more lung metastases and liver metastases in the WT mice than that in Erbin knockout mice (data not shown). This evidence concerns the gene ERBIN and malignant colon neoplasm.