PTPRC and colorectal carcinoma: To study the components and dynamics of B cells in lung metastasis of CRC, we established a mouse model for lung metastasis of CRC and isolated B220+ cells in tumors using BD IMag Mouse B Lymphocyte Enrichment Kit and analyzed these cells using single-cell RNA sequencing (scRNA-seq) involving a single-tube protocol with unique transcript counting through barcoding with unique molecular identifiers (UMIs).