Then, we used flow cytometry to label and analyze the number of CCR3+, CCR9+, CCR10+, CXCR2+, CXCR5+, and CXCR6+ cells expressed different isotype antibodies including IgA, IgG, and IgM in the lung metastases of cKO and control tumor-bearing mice. The gene discussed is CD40LG; the disease is neoplasm.