We performed these markers at the first round of IHC and it was positive for CK7, TTF-1, galectin 3 and napsin A, and negative for CK20 and GCDFP-15 (Supplementary Fig. 3) TTF-1 and napsin A are very important markers for the lung cancer diagnosis, GCDFP-15 is important for breast cancer, and CK7, galectin 3, and TTF-1 are important for thyroid cancer diagnosis. The gene discussed is TTF1; the disease is breast carcinoma.