To confirm the inhibitory effect of N1IC-GFP/MAF is caused by the Notch1 activation, we employed a dominant-negative mutant of Mastermind-like 1 (DN-MAML-1) to inhibit activated Notch pathway in N1IC-GFP/MAF, and then tested effect of the conditioned medium (CM) derived from DN-MAML-1/N1IC-GFP/MAF vs. Mock/N1IC-GFP/MAF on growth of melanoma cells. The gene discussed is MAML1; the disease is melanoma.