We then examined the possible induction of WISP-1 (WNT1-inducible-signaling pathway protein-1) expression in N1IC-GFP/MAF, as we had previously found that Notch1 activation upregulates WISP-1 expression and WISP-1 is responsible for mediating inhibitory effect of stromal fibroblasts carrying high Notch1 pathway activity on melanoma growth [11]. The gene discussed is CCN4; the disease is melanoma.