Mice were injected intraperitoneally with a dose of 2×107 live leptospires, weighed individually and blood sampled at defined time-points post-infection (p.i.)to observe and measure the early production (day (D)3, D8 and D15) of anti-Leptospira immunoglobulins (Igs) in serum and their evolution (D30, D60, D90 and D120) and long-term persistence up to 6 months p.i. (D180). Here, CUBN is linked to infection.