The cellular material was derived from four healthy controls and two ALS patients with VCP mutations: R155C and R191Q, hereafter termed VCPmu (Fig. 1A) [95 samples from six time points and two genotypes (healthy and VCPmu-related ALS); four clones from four different healthy controls and three clones from two VCPmu patients]. This evidence concerns the gene VCP and amyotrophic lateral sclerosis.