MAPT and early-onset autosomal dominant Alzheimer disease: Immunoblot analysis of sarkosyl-insoluble fractions with T46, which recognizes the C-terminal tau (404–441), and pS396 tau antibodies showed that different tau isoforms accumulated in each tauopathy: there were two major bands at 60 and 64 kDa in PiD, two major bands at 64 and 68 kDa in PSP and CBD, and three major bands at 60, 64 and 68 kDa in Alzheimer’s disease, which represent hyperphosphorylated full-length tau proteins (Fig. 1A and Supplementary Fig. 1).