Genome-wide linkage analysis showed the association of TBX22 role in the development of NSCLP.74In addition, mutations in TBX22 were found in individuals with isolated CPO.75, 76, 77It plays a significant role in tooth and upper lip development and causes hypodontia and cleft lip.78DNA methylation study suggests that cleft palate-susceptible gene Tbx22 is associated with gene expression and might be responsible for the developmental failure of palatal fusion, eventually resulting in the formation of cleft palate.79 This evidence concerns the gene TBX22 and cleft palate.