act-EGFR mutant allele frequency (MAF) and T790M/act-EGFR MAF ratio; 36 cancer-related genes; TP53, RB1, BRAF, KIT, NOTCH1–4, PIK3CA, PTEN, FGFR1, MYC, MYCL1, and MYCN. Here, RB1 is linked to cancer.