TJP1 and infection: For example, Briceño et al. (2016) used Caco-2 cells and reported the redistribution and decrease of Zo-1 after 24 h post infection (p.i.)whereas studies in MDCK (Barragan et al., 2005), m-ICc12 cells (Weight et al., 2015) and Caco2 or murine brain endothelial cells (Ross et al., 2019) showed no alterations in the distribution of this protein after 2 to 6 h p.i. With the longer time points of these studies in mind, we extended TEER measurements, RT-qPCR, and the IFA of tight junction components to 48 h.