To assess the roles of MLK3 in the biological behaviors of glioma cells, we first detected the protein levels of MLK3 in high-migration glioma cell lines (U87, U118, and U251) and low-migration glioma cell lines (U343 and T98G) 48 h after serum starvation (Figure 3A). The gene discussed is MAP3K11; the disease is central nervous system cancer.