Cross-validated SALL2 target genes from GBM, our ChIP-seq studies in HEK293 cells, and the available gene expression profiling for SALL2 identified a core set of genes, including PODXL and NRARP. Consistently, a positive relationship between SALL2 and PODXL expression is found in several tissues, including brain and colon cancers, and PODXL expression is perturbed upon CRISPR/Cas9-mediated SALL2 knock-out in a human epithelial colon cell line. This evidence concerns the gene PODXL and malignant colon neoplasm.