CD4 and metabolic dysfunction-associated steatotic liver disease: We first performed CyTOF to determine the frequencies of the major immune cell subsets (NK cells, B cells, CD4+ T cells, CD8+ T cells, monocytes, mDC1s, and pDCs) in the peripheral blood of 13 HVs and 42 NAFLD patients, of whom 27 had mild or no fibrosis (F0–2) and 15 had advanced fibrosis (F3–4).