We first performed CyTOF to determine the frequencies of the major immune cell subsets (NK cells, B cells, CD4+ T cells, CD8+ T cells, monocytes, mDC1s, and pDCs) in the peripheral blood of 13 HVs and 42 NAFLD patients, of whom 27 had mild or no fibrosis (F0–2) and 15 had advanced fibrosis (F3–4). Here, CD8A is linked to metabolic dysfunction-associated steatotic liver disease.