While 196hT cells do not express tenascin-W when cultured alone (Figures 4A, B), the tenascin-W labeled by immunocytochemistry in co-cultures of Huh-28 with 196hT cells is organized in fibrils surrounding nests of Huh-28 cells, mimicking the appearance of tenascin-W in the stroma surrounding nests of tumor cells in ICC. Here, TNN is linked to intrahepatic cholangiocarcinoma.