Studies have also confirmed that MIF plays a crucial role in myocardial protection by ischemic preconditioning (Ruze et al., 2019), Ischemic preconditioning activates RISK and AMPK signal pathways through MIF, reduced myocardial infarction area and cardiomyocyte apoptosis (Dow et al., 2012; Emontzpohl et al., 2019), inhibition of ROS production (Yang et al., 2010) and inflammatory infiltration of cells, maintained endothelial cell function (Argaud et al., 2005), thereby improving cardiac dysfunction caused by myocardial I/RI (Vinten-Johansen et al., 2007). This evidence concerns the gene MIF and myocardial infarction.