For the further mechanism studies, the target genes that efficiently bound to miR-483-5p were TIMP2 and MAPK1, and this conclusion was also corroborated by an RNA pull-down assay, hinting that miR-483-5p might participate in the regulation of type 1 and type 2 diabetes through TIMP2 and MAPK1. The gene discussed is MAPK1; the disease is type 2 diabetes mellitus.