In order to investigate the roles of OSM and IL-11 in ICC cells, we analyzed the phosphorylation of Akt, ERK1/2, p38 MAPK, JNK, p65 (nuclear factor kappa B pathway) and STAT3 in ICC cells treated with or without OSM (5 ng/mL) and IL-11 (10 ng/mL) to determine the kinase profiles of distinct signaling pathways. The gene discussed is MAPK8; the disease is intrahepatic cholangiocarcinoma.