MLC1 and infection: However, LLC-PK cells pretreated for 1 h with RhoA inhibitor (CT04), ROCK inhibitor (Y27632), or MLC inhibitor (blebbistatin) showed pronounced reductions in the transepithelial leakage of FD4 and FD70 induced by PSaV infection in the presence of GCDCA (15- to 16-fold decrease in FD4 permeability or 4- to 5-fold decrease in FD70 permeability) or in the absence of GCDCA (13- to 14-fold decrease in FD4 permeability or 3- to 4-fold decrease in FD70 permeability) (Fig. 12A and B).