Thus, we used RNA FISH to detect the subcellular localization of ZNF582-AS1 in OSRC2 and Caki-1 cell lines, and the RNA FISH results showed that ZNF582-AS1 was distributed mostly in the cytoplasm of ccRCC cells (Fig. 1g). This evidence concerns the gene ZNF582 and nonpapillary renal cell carcinoma.