SPIB and primary biliary cholangitis: The SNP, rs11546996, a SNP associated with primary biliary cirrhosis, was a methylation-adjusted eQTL for PNKP. In a previous GWAS study, a causal SNP-gene association for primary biliary cirrhosis was found with rs11546996 and the causal gene was assumed to be SPIB, as it is the closest gene.23 Since our study specifically looked at gene expression in hepatocytes, a tissue relevant for this disease, we may have found a potentially novel SNP-gene pair associated with primary biliary cirrhosis whose expression is regulated by both DNA methylation and gene variation.