GRIA2 and infection: Given the higher infection rate of TKIT-SEP-GluA2 AAV compared to in utero electroporation, we were able to visualize a higher density of SEP-GluA2 expressing neurons which will allow the longitudinal study of synaptic surface GluA2 on multiple neurons within one field of view, with KI sparse enough to distinguish individual synaptic puncta and neuronal processes.