This suggested that TGF‐β1‐induced LX‐2 cells were associated with the up‐regulation of α‐SMA, Col1 and Col3, which are among the important characteristics and markers for HSC activation and liver fibrosis, and TGF‐β1 successfully induced the activation of HSC cells in vitro. This evidence concerns the gene TGFB1 and Hepatic fibrosis.