Thus, to further investigate the TF function of RELAFUS1, we focused on the LMX1B gene, a brain-developmental transcription factor, which was one of the common RELAFUS1 target genes in 293T-RELAFUS1 and mouse ependymoma cells and was also selected as one of the enhancer-associated genes specific for human RELAFUS tumors and mouse ependymoma cells (Fig. 2k, 5a) [31]. RELAFUS1 bound similar positions on the LMX1B gene locus in 293T and mouse ependymoma cells. Here, LMX1B is linked to ependymoma.