EN2 and colorectal carcinoma: MTT assay, Transwell experiments, and Western blot showed that circ_0000467 knockdown impeded the multiplication, migration, invasion, and EMT of SW620 and HT29 cells while the suppressing effects of circ_0000467 knockdown on CRC migration, invasion, and EMT process were partially abolished after EN2 overexpression (Figure 5(b-d)).