We collected RNA from J2315-infected iBMDMs early in infection and ran RT-PCR for both Rnf31 and Rbck1 (also known as HOIL-1, another key member of LUBAC) and found that both were transcribed at significantly greater levels in WT cells compared to Ifnar1-/- cells, supporting the difference found in the RNAseq dataset (Fig 5A). This evidence concerns the gene IFNAR1 and infection.