In order to ensure that the LPS-TLR4-IFN paradigm found for many Gram-negative pathogens holds during cellular infection with Burkholderia cenocepacia, we infected differentiated THP-1 cells, a human monocyte/macrophage cell line (Fig 1A), as well as murine bone marrow-derived macrophages (BMDMs, Fig 1B) with J2315, a common laboratory strain of Bc, and measured IFNB1/Ifnb1 transcription (Fig 1A and 1B) as well as that of the canonical ISGs Rsad2 and Cxcl10 (Fig 1B). Here, RSAD2 is linked to infection.