By stimulating WT and Ifnar1-/- BMDMs with either J2315 or Kdo2-Lipid A (KLA, the immune-stimulatory portion of LPS) and collecting RNA at various time points for RNA sequencing, we aimed to better understand the nature of the IFN-dependent transcriptional response to cytosolic Gram-negative bacterial infection and, especially, how it may differ from the LPS response. Here, IFNA1 is linked to gram-negative bacterial infections.