For example, there are many preclinical and clinical studies demonstrating the existence of ESR1 mutations in primary tumors and metastasis lesions, which significantly promoted breast cancer progression [44]; BUB1 was reported to act as an important biomolecule in the regulation of cell cycle in colorectal cancer [45,46].In another word, our credibility of bioinformatic analyses was partially enhanced by the aforementioned publications. The gene discussed is BUB1; the disease is colorectal cancer.