ANO1 and gastrointestinal stromal tumor: It is noteworthy that TMEM16A knockdown resulted in 96% inhibition of chloride ion outflow in GIST-T1 and 90% inhibition in GIST882 by whole-cell patch-clamp experiments, indicating that TMEM16A is a key regulator for GIST cells to balance chloride balance (Simon et al., 2013).