In line with the western blot analyses (Figure 2C), immunofluorescence studies showed lysosomal localization of all PD-associated CTSD variants as indicated by co-localization with lysosomal marker LAMP2 (Figure 2F and Supplementary Figure 4), also confirmed by Pearson’s correlation analysis assessing overlay of LAMP2 and CTSD signal (Supplementary Figure 5A). The gene discussed is CTSD; the disease is Parkinson disease.