To confirm the direct binding between miR-5001-5p and HES6, pmirGLO-HES6-3′UTR construct (WT HES6) or mutant derivatives lacking the putative miR-5001-5p recognition sequence (MUT HES6) were transfected into CRC cell lines together with either miR-5001-5p mimics or miR-5001-5p inhibitors. The gene discussed is HES6; the disease is colorectal carcinoma.