TIL micro-cultures displaying tumor reactivity against HLA-matched or autologous tumor cells are selected and expanded further in the REP stage under an allogeneic feeder co-culture with healthy donor irradiated (40 Gy) peripheral blood mononuclear cells (PBMC) in a 1:200 ratio with 30 ng/ml anti-CD3 (clone OKT3) and 6,000 IU/ml IL-2. The gene discussed is IL2; the disease is neoplasm.