The colonies have distinct borders and sharp edges.3,15,17 The derived iPSCs should be positive for the expression of pluripotency markers like OCT4, SOX2, NANOG, LIN28, SSEA-3, SSEA-4, TRA-1-60.21 The iPSCs derived by the episomal reprogramming of T cells from Pakistani Duchenne Muscular dystrophy patients were morphologically similar to the previously published data. Here, POU5F1 is linked to Duchenne muscular dystrophy.