To determine the effect of CCR6 deficiency on B cell subset distribution in PVAT and other tissue compartments in atherosclerosis-prone mice at homeostasis, flow cytometry and ELISPOT experiments were performed in young ApoE−/−CCR6+/+ and ApoE−/−CCR6−/− littermate control mice (10 weeks old) fed normal Chow diet. The gene discussed is CCR6; the disease is atherosclerosis.