TNF and infection: Next, to evaluate whether asymptomatic infection with HSV-1 modulates the cytokine environment in the CNS upon EAE induction, we performed RT-qPCR for a set of cytokines that either, promote an inflammatory state in this tissue (i.e., IL-1β, IL-6, TNF-α and IFN-γ) or an anti-inflammatory environment (i.e., IL-10).