BRD4 and serum lipopolysaccharide activity: Because of the suppression effects of JQ1 on inflammation cytokines IL6, IL18, and IL1β in endotoxemia colons, we further confirmed effects of BRD4 inhibition on the phosphorylation of NF-κB by western blot, and found the upregulated expression of phosphorylated NF κB in endotoxemia colon was obviously reduced by JQ1 pretreatment (Figure 4A).