Since pyroptosis is a novel proinflammatory form of cell death that often occurred in inflammatory pathogenesis, we next confirmed the pyroptosis in endotoxemia colon, and interestingly discovered the activation of NLRP3/ASC/caspase 1 complex and even the elevation of pyroptosis markers—gasdermins family such as GSDMD, GSDME, and GSDMA. Here, GSDMD is linked to serum lipopolysaccharide activity.