Moreover, Alflen et al. did not formally evaluate the roles of NETs in the aspergillosis by determining whether (1) NETs were evident in the lung tissues of wild-type or PAD4-KO mice, (2) NETs were formed by wild-type or PAD4-KO neutrophils or whether (3) DNAse treatment had an effect in the outcome of infection (Alflen et al., 2020). This evidence concerns the gene PADI4 and infection.