To assess whether adult PS1-KI or 3xTg-AD astrocytes influence neuronal viability, we treated DIV15 neurons with CM from each astrocyte type (C57BL6, PS1-KI and 3xTg-AD) (Figure 2A), quantified the percentage of condensed nuclei and evaluated neuron morphology. This evidence concerns the gene PSEN1 and Alzheimer disease.