PD-L1 expression was prospectively determined in patients with solid tumors using the Ventana PD-L1 (SP263) assay, in which expression levels were classified as PD-L1 high (if ICs involve > 1% of the tumor area, TC ≥ 25% or IC ≥ 25%; if ICs involve < 1% of the tumor area, TC ≥ 25% or IC = 100%) or PD-L1 low/negative (84, 85). This evidence concerns the gene CD274 and neoplasm.