The CheckMate 275 clinical trial, studying nivolumab as a first-line treatment of patients with metastatic or surgically unresectable urothelial carcinoma, determined PD-L1 expression at screening of patients using the Dako PD-L1 IHC 28-8 pharmDx kit, though this was not used as inclusion criteria (63). This evidence concerns the gene CD274 and urothelial carcinoma.