Furthermore, in this study, the tumor origin of the liver‐specific mRNA, such as AFP, ALB, GPC3 and EpCAM were not confirmed in the clinical samples, because the MCA system was specifically programmed for CTC detection and identification, and only the three antibodies including CK antibody, CD45 antibody, and DAPI provided by Hitachi Chemical were available in this method. This evidence concerns the gene GPC3 and neoplasm.