To overcome the limited translatability of established breast cancer cell lines and corroborate the role of the TGF-β/AURKA oncogenic axis in driving cancer plasticity and chemoresistance in clinically relevant models, we established unique TNBC-M14 and TNBC-M25 (Supplementary Fig. 5c) isolated from patient-derived TNBC brain metastasis. Here, TGFB1 is linked to breast carcinoma.